Immuno-electro-diffusion on cellulose acetate membrane for serological testing in human malaria
Druilhe, P.; Monjour, L.; Richard-Lenoble, D.; Gentilini, M.
Pathologie-Biologie 26(3-4): 169-172
1978
ISSN/ISBN: 0369-8114 PMID: 98748 Document Number: 131477
An immuno-electro-diffusion assay for human malaria, using cellulose acetate gel, is described. More than 3500 sera have been tested by this method, which proved very useful for epidemiology since 400 sera a day can be tested by one person, with simple equipment. Its sensitivity compared well to the IFA test, although the antibodies revealed are quite different. A constnat precipitin line appeared, against 56 degrees C labile antigens, in sera of subjects infected by P. falciparum, and up to 8 other precipitins per serum tested. The test may be useful in analyzing these multiple antibodies occurring in patients with malaria.