Inactivation of Herpes Simplex Virus type 1 & Adenovirus Type 5 by Direct Electric Current at a Biocompatible Level in Vitro
Roohandeh, M.; Bamdad, T.
Clinical Laboratory 57(7-8): 489-495
2011
ISSN/ISBN: 1433-6510 PMID: 21888012 Document Number: 13069
Background: In vitro studies were conducted to quantify the effectiveness of low-level direct electric current (DC) on infectivity of Herpes Simplex Virus type 1 (HSV-1), Adenovirus type 5 (AdV-5), and on viability of Vero cells. Methods: Both viruses and Vero cells were exposed to DC by using platinum electrodes connected to a DC source, then the viral infectivity and cell viability were assessed by plaque and MTT assay, respectively. Results: The results showed that both viruses were inactivated completely by 200 μA DC in 10 minutes (current density = 20 μA/mm 2 ) while this amount of DC had no significant changes on the viability of Vero cells (viability > 90 %). Inactivation degree of HSV-1 and AdV-5 was 5 and 4 log per mL, respectively. Further study is required to investigate the mechanism of inactivation by this method. Conclusions: DC at a biocompatible level showed the competency to inactivate the viruses in the solution completely. So it is a useful tool for designing a noninvasive method for decontamination of biological or synthetic fluids. This method or derivation can be considered as an easy, fast, and economical method for fluid decontamination.
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