Studies on heterogeneity of the lysine-rich histone from ox pancreas
Lipińska, A.; Kłyszejko-Stefanowicz, L.
Acta Biochimica Polonica 25(2): 157-162
1978
ISSN/ISBN: 0001-527X PMID: 569416 Document Number: 130177
1. Histone H1 from ox pancrease has been isolated by preparative electrophoresis at pH 2.7 in polyacrylamide slab gel, using the fraction F1 of Oliver et al. (1972, Biochem. J., 129, 349--353) as starting material. 2. The isolated histone H1 showed higher heterogeneity on isoelectric focusing than on polyacrylamide electrophoresis in long gel. The isoelectric points of the main subfractions of histone H1 were at pH 8.0--8.4.
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