Column extraction analysis in pharmacology and toxicology
Breiter, J.
Arzneimittel-Forschung 28(11a): 1941-1944
1978
ISSN/ISBN: 0004-4172 PMID: 582522 Document Number: 129929
The extraction of lipophilic compounds from body fluids is preferably performed with a new procedure, the Extrelut-column extraction. The aqueous solution is applied onto the column and remains as the stationary phase on the porous support material. On elution with organic solvents lipophilic substances are extracted from the aqueous phase into the eluent. The method offers several advantages: No formation of emulsions, saving of solvents, high recovery rates and purer extracts than are obtained using conventional extraction. Three examples of application are described: A barbiturate determination from blood and serum, which is highly simplified compared with the conventional extraction. The isolation of basic compounds from blood using extraction and simultaneous purification by means of coupled extraction and absorption columns. A thin-layer chromatographic screening procedure for drugs in urine including column extraction.