Passage of intact IgG from plasma to the oral cavity via crevicular fluid
Challacombe, S.J.; Russell, M.W.; Hawkes, J.
Clinical and Experimental Immunology 34(3): 417-422
1978
ISSN/ISBN: 0009-9104 PMID: 105828 Document Number: 128146
The aim of this investigation was to determine whether IgG could pass from the blood to the oral cavity. Pure IgG was prepared from monkey serum by ion exchange chromatography and gel filtration and was radiolabeled with 125I. This was injected i.v. into 8 rhesus monkeys. Radioactivity could be detected in crevicular fluid washings, and in mixed and parotid saliva samples 30 min after injection. Ultracentrifugation on sucrose density gradients revealed that most of the radioactivity in crevicular fluid washings was associated with proteins having a sedimentation coefficient similar to marker IgG. Radioactivity in parotid saliva was not found in the IgG zone, but was present in zones with sedimentation coefficients of approximately 4.5 S and 1 S. IgG may pass as an intact molecule from plasma to crevicular fluid. Serum antibodies could play a role in protection against dental caries.