Specific splitting of alpha-tropomyosin at cysteine-190 and a physico-chemical study of the fragments
Potekhin, S.A.; Filimonov, V.V.
Biofizika 23(6): 986-990
1978
ISSN/ISBN: 0006-3029 PMID: 719038 Document Number: 126626
alpha-tropomyosin from rabbit spine muscle specifically cleaves at cystein-190. A study has been made of the physico-chemical parameters of the fragments resulting from the cleavage. The distortion of their structure by the cleavage is not great. The N- and C-end fragment are able to form a complex with an association constant of 5.10(4) M(-1). It is shown electrophoretically that troponin binds with the fragments only in the case when both fragments are present in the solution.