The importance of ionic strength and protein concentration in the dialysis performance of autoanalyzer systems for the enzymatic determination of uric acid
Hunziker, P.; Keller, H.
Journal of Clinical Chemistry and Clinical Biochemistry 16(4): 249-251
1978
ISSN/ISBN: 0340-076X PMID: 641453 Document Number: 126377
An earlier method for the determination of uric acid (this journal 13, 89--96, (1975) was reevaluated. It was confirmed that it is necessary to add NaCl and a detergent to the wash fluid in order to obtain full sensitivity and linearity. Protein components in the samples or in secondary standard solutions also increase sensitivity and straighten the calibration curve of primary standard solutions. The effects are compared with results reported in the literature, and the possible mechanisms of these effects are discussed.