Sequential demonstration of nucleic acids and protein fractions in the same cells
Erenpreĭsa, E.A.
Arkhiv Anatomii Gistologii i Embriologii 72(2): 95-97
1977
ISSN/ISBN: 0004-1947 PMID: 857779 Document Number: 123934
Some combinations are suggested to reveal cell components in the following sequence: 1) RNA--DNA--total proteins; 2) RNA--DNA--histones; 3) RNA--DNA--histones--total proteins; 4) RNA--DNA--acidic proteins. RNA is stained with pyronin Y (in the mixture with methyl green). DNA--by the Feulgen procedure (with TCA hydrolysis), histones and total proteins--by Alfert--Geschwind's method and acidic proteins--according to the technique of Smetana and Bush. The details of the whole procedure, adopted for formol-fixed smears are described.