Serum glucose, insulin, total, pancreatic and enteric glucagon in dogs before and after pancreatectomy, in patients with gastrectomy and pyloroplasty and in diabetic children. Effects of insulin and of secretin
Matsuyama, T.; Hoffman, W.H.; Breuer, R.I.; Zuckerman, L.; Lawrence, A.M.; Foa, P.P.
Materia Medica Polona. Polish Journal of Medicine and Pharmacy 9(2): 124-129
1977
ISSN/ISBN: 0025-5246 PMID: 895187 Document Number: 123266
After pancreatectomy in 8 dogs plasma immunoreactive insulin (IRI) fell and plasma glucose, glucagon-like immunoreactive material (GLI), pancreatic glucagon (PG) and enteroglucagon (EG) rose. When 5% glucose solution was injected into the ileum there was a greater rise in GLI than when glucose was injected into the jejunum but IRI did not change. The GLI response to glucose increased progressively after pancreatectomy and GLI increments were represented entirely by the EG fraction. The effect of pancreatectomy on plasma GLI was partly reversed by insulin sufficient to cause a significant rise in plasma IRI but only a moderate fall in plasma glucose.Glucose 100 g was given by mouth, sometimes with secretin by vein, to 7 healthy subjects, 5 patients after gastrectomy and 7 patients after pyloroplasty. Fasting plasma glucose, IRI and GLI of patients did not differ from those of controls but after glucose load were greater for patients; PG did not change or tended to fall in controls and patients with pyloroplasty but rose in patients with gastrectomy. Secretin partly corrected the abnormalities in gastrectomized patients but not those in patients with pyloroplasty and had no effect on PG except in those with gastrectomy for whom it rose. In 10 diabetic children with low values for IRI, PG and EG were increased. Insulin treatment reduced both glucagon fractions to values similar to those in 23 healthy children. PG was only slightly increased and EG not at all in 3 diabetic children with normal IRI. It is concluded that GLI has a role in the regulation of carbohydrate metabolism and abnormalities in GLI secretion may contribute to the glucose intolerance in experimental and human diabetes and after gastrectomy and pyloroplasty.