Analysis of substances released from Zajdela ascites hepatoma cells exposed to UV radiation of different wavelengths. III. Release of nucleoproteins and carbohydrates
Krylenkov, V.A.; Samoĭlova, K.A.; Tret'iakov, A.V.
Tsitologiia 19(8): 894-899
1977
ISSN/ISBN: 0041-3771 PMID: 199969 Document Number: 122575
Irradiation of the Zaidela ascite hepatoma cells with physiological doses of shortwave length (254 nm) and longwave length (300-380 nm) UV light (far and near UV radiation) is accompanied by the release of ribonucleoproteins (RNP) from the cells, whose amounts increase with dose. Irradiation with far and near UV light leads to the release of high-molecular and low-molecular RNP, respectively. No deoxyribonucleoprotein were found among the released substances. Non-protein fractions, released from irradiated cells, contain carbohydrate-like substances. At maximum far and near UV doses the amounts of these substances constitute 180-190% of the control and 6% of their amount in intact cells. After irradiation with far UV light, relatively high-molecular carbohydrates are released, while near UV light treatment induces the release of low-molecular carbohydrates. The criteria tested show that the efficiency of far UV light exceeds that of near UV light by one order.