RNA biosynthesis in the ascitic cells of Ehrlich's carcinoma and Zajdela's hepatoma under conditions of blocked oxidative phosphorylation
Shilov, L.A.
Voprosy Onkologii 23(9): 55-59
1977
ISSN/ISBN: 0507-3758 PMID: 198951 Document Number: 115835
In experiments in vitro on ascites tumor cells of Ehrlich carcinoma and Zajdela hepatoma the author studied the effect of 2,4-dinitrophenol (an agent dissociating respiration from phosphorylation) on respiration, glycolysis, resynthesis of ATP and synthesis of basic fractions of cytoplasmic RNA by the incorporation of labeled 3N-uridine precursor. It was shown that under optimum conditions of tumor cell incubation (phosphate-rich Igle medium) in the presence of 6.10(-4) M DNP a sharp activation of anaerobic glycosis is observed as well as increased O2 absorption and high level of ATP. Blocked phosphorylation associated with respiration renders no appreciable effect on the biosynthesis of basic fractions (4 S, 18 S, 28 S) of cytoplasmic RNA.