Participation of lymphoid organ T- and B-lymphocytes in transplantation immunity in immune deficiency modeled with anti-thymus serum and cyclophosphamide
Antonenko, V.T.; Chornen'ka, V.D.
Fiziolohichnyi Zhurnal 23(6): 733-740
1977
ISSN/ISBN: 0015-3311 PMID: 338369 Document Number: 121935
Allotransplantation of skin from C57Bl mice to CBA mice decreased the level of T-lymphocytes were observed. In simulating T-lymphocyte deficiency using rabbit antithymus serum concurrent with skin transplantation, the level of T-lymphocytes, sensitive to antibrain serum, decreased in the thymus, lymph nodes and spleen on the 3rd and, especially, on the 7th day; B-lymphocyte levels decreased on the 7th day in lymph nodes and spleen, resulting in prolonged viability of the allotransplant. Administration of cyclophosphamide during the inductive phase of transplantation immunity formation created a B-lymphocyte deficiency in lymph nodes and spleen on the 3rd and 7th day after transplantation and increased T-lymphocyte levels on the 7th day. The increase in allotransplant viability indicated the participation of the B system in transplantation immunity formation. Combined administration of antithymus serum and cyclophosphamide concurrent with allotransplantation significantly decreased B-lymphocyte levels in the spleen and lymph nodes without affecting T-lymphocyte levels and prolonged allotransplant viability. The simulation of deficiency of the T-dependent system prolonged the viability of skin allotransplants in comparison with deficiencies in the B-dependent immunity system.