Stability of 2-chloro-2'-deoxyadenosine at various pH and temperature

Tarasiuk, A.; Skierski, J.; Kazimierczuk, Z.

Archivum Immunologiae et Therapiae Experimentalis 42(1): 13-15

1994


ISSN/ISBN: 0004-069X
PMID: 7503627
Document Number: 1219
In this paper data concerning the stability of 2-chloro-2'-deoxyadenosine (2-CdA) solutions at various pH and temperature conditions are presented. Decomposition of 2-CdA was measured as content of its hydrolysis product - 2-chloroadenine, using the HPLC method. We found that 2-CdA is stable at basic and neutral pH and temperatures between 37 and 80 degree C. At acidic pH. decomposition markedly increased with time, even in physiological temperatures. At pH 2 and 37 degree , after 6 h. only 13% of 2-CdA remained in solution. At pH 1 - after 2 h of heating to 37 degree 2-CdA content was as low as 2%. Calculated half time T-1/2 was equal to 1.6 h (pH 2, temperature = 37 degree ) and 0.37 h (pH 1, temperature = 37 degree ).

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Stability of 2-chloro-2'-deoxyadenosine at various pH and temperature