Study of the glucocorticoid receptors of the cytosol in various organs of the rat
Volchek, A.G.; Danilov, S.M.; Kim, A.A.
Problemy Endokrinologii 23(3): 94-100
1977
ISSN/ISBN: 0375-9660 PMID: 887638 Document Number: 116080
A method of hard phasic separation of free and bound forms of the hormone demonstrated the presence of specific (saturated) and nonspecific (unsaturated) binding of dexamethazone in the cytosol of various rat organs; and increase of the sorbent concentration promoted a better detection of the first type of binding on account of selective depression of binding of the second type. Cortisol was bound with dexamethazone receptors of various rat organs in the binding sites common with dexamethazone, replacing the latter from its complex with the receptors. The constant of dexamethazone association with its receptors in the brain, liver lungs, spleen, thymus, and kidney cytosol was of the order of 10(8) M-1, with the binding capacity of cytosol of these organs within the range of from 0.6 to 4 ng hormone per 1 g of raw weight.