Characterization of cytosol and nuclear glucocorticoid receptors from rat liver tissue in hepatocarcinogenesis
Krauialis, K.I.; Ulinskaĭte, A.P.; Kil'dema, L.A.; Diashrius, A.P.; Iurkshaĭtite, R.I.
Voprosy Meditsinskoi Khimii 29(3): 111-116
1983
ISSN/ISBN: 0042-8809 PMID: 6880113 Document Number: 204549
Specific receptors with high affinity for dexamethasone (Kass = 2.03 = 4.04 . 10(8) M-1) were found in liver cytosole of control rats as well as in animals with hepatocarcinogenesis caused by diethylnitrosamine and in cytosole of primary hepatomas. Amount of the binding sites for dexamethasone varied depending on severity of the blastomatous process (3.4 = 0.17 X X 10(-13) M/mg protein). The receptors from liver tissue of control rats, malignant liver and hepatomas were similar in their binding patterns and in chromatigraphic properties. Content of dexamethasone-receptor complexes accepted by chromatin was decreased by 50% in cytosole of malignant liver cells as compared with controls. The same alteration in binding of cytosole-receptor complexes of DNA from malignant liver tissue was found by means of affinity chromatography.