Isolation of living adult Onchocerca volvulus from nodules
Schulz-Key, H.; Albiez, E.J.; Büttner, D.W.
Tropenmedizin und Parasitologie 28(4): 428-430
1977
ISSN/ISBN: 0303-4208 PMID: 203061 Document Number: 115807
A technique for the isolation of adult Onchocerca volvulus from excised onchocercomata is described. The nodules are incubated in medium 199 containing 1-5 mg collagenase and 0.2 mg gentamicin per ml for 6-48 hours in a waterbath at 30-37 degrees C. A proportion of the worms can be isolated alive.
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