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Identification of CD phage proteins participating in DNA-protein interaction by means of O-methylhydroxylamine

Kiseleva, N.P.; Zinchenko, A.I.; Khromov, I.S.; Tikhonenko, T.I.

Biokhimiia 42(6): 1090-1096

1977

ISSN/ISBN: 0320-9725
PMID: 889973
Document Number: 115115
An early described hypothetical scheme is confirmed on transformation of non-covalent polynucleotide-protein interactions into covalent under the treatment of CD phage with O-methylhydroxylamine. 4 proteins are revealed, which have been covalently bound to cytosine of DNA during modification process. Fractionation of these proteins in polyacrylamide gel in the presence of sodium dodecylsulphate has revealed that three of them migrate as head structural proteins, and the rest--as internal phage CD protein. Possible mechanisms of the effect of phage protein on the reaction of DNA cytosine residues with O-methylhydroxylamine are discussed.

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