Lambdoid phage structural proteins and antigens
Ivanov, V.N.; Andreev, I.L.; Reshetnikov, S.S.; Mertvetsov, N.P.
Molekuliarnaia Biologiia 13(3): 519-530
1979
ISSN/ISBN: 0026-8984 PMID: 379613 Document Number: 146392
The composition of structural proteins of lambdoid phages such as .lambda., .vphi. 80 and 434 divided by MW was determined by SDS-disc-electrophoresis in a 15% polyacrylamide gel. The proteins of the same phages were divided by isoelectric points using an isoelectric focusing in a 5.25% polyacrylamide gel with 8 M urea and a gradient pH 7.0-3.5. Both methods brought out a composition character of the virion proteins and illustrated the high degree of similarity among the structural proteins of phages .lambda. and 434 and far less of a similarity among the proteins .lambda. and .vphi. 80. The antigenic composition of the lambdoid phage was determined and the basic antigens were identified on 1-dimensional and 2-dimensional immunoelectrophoregrams. The appreciable immunochemical affinity of basic antigens of the phages .lambda. and 434, but a partial affinity of the phages .lambda. and .vphi. 80, were found. The basic protein of the head pE was immunochemically similar for all 3 phages.