Lipoprotein analysis by means of the fluorescence probe 8-anilino-1-naphthalene sulphonic acid
Koller, E.; Vukovich, T.; Koller, F.; Doleschel, W.
Wiener Klinische Wochenschrift 89(11): 379-382
1977
ISSN/ISBN: 0043-5325 PMID: 878479 Document Number: 114803
Single and mixed lipoprotein fractions were successfully analyzed by utilizing the fluorescent propertby of the applied reagent, 8-anilino-naphthalene sulphonic acid. The described method is especially useful for determination of lipoproteins present in high dilution, as found in chromatographic column effluents. The concentration dependence of the developed fluorescence was determined and this served as basis for quantitation. Furthermore, the measured activation spectra show two peaks for each lipoprotein class, the relative intesities being different for each class. The intensity quotient shows a tendency towards linear concentration dependence. Investigation of binary and ternary mixtures showed that the ratio of the two maximum intensities is additive, in contrast to the intensities themselves.