Isolation and preliminary characterization of temperature-sensitive mutants of pseudorabies virus
Huy, V.D.; Stäber, H.; Waschke, K.; Rosenthal, H.A.
Acta Virologica 21(5): 397-404
1977
ISSN/ISBN: 0001-723X PMID: 22234 Document Number: 114786
Nine ts mutants of pseudorabies virus (PRV) strain BUK were isolated following bromodeoxyuridine (BUdR) or NaNO2 mutagenesis by a selection technique based on a shiftdown from nonpermissive to permissive temperature. This technique is described and its advantages are discussed. The nine ts mutants have been assigned to four complementation groups.
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