The nature of a cytopathogenic material present in amebae of the genus Naegleria
Dunnebacke, T.H.; Schuster, F.L.
American Journal of Tropical Medicine and Hygiene 26(3): 412-421
1977
ISSN/ISBN: 0002-9637 PMID: 869097 Document Number: 114321
The Naegleria amoeba cytopathogenic material (NACM) obtained from free-living and/or pathogenic amoebae [N. gruberi, N. jadini, N. fowleri, Acanthamoeba] was partially isolated and characterized. The evidence indicates that the material is a protein or has a protein component, and is in the estimated molecular weight range of 50,000 daltons. The partially isolated NACM, like its counterpart in the amoebic lysates, is capable of sustaining itself in tissue cultures at a temperature which otherwise would inactivate it, and in serial passages through multiple dilutions. No distinctions between the NACM from amoebae of different Naegleria spp. or strains were found in the biological response to the various treatments studied.