An improved procedure for purifying RNA tumor viruses from malignant tissue
Gulati, S.C.; Scholes, J.; Weiss, M.; Baldi, A.; Zeelon, E.; Spiegelman, S.
Acta Physiologica Latino Americana 27(4): 183-187
1977
ISSN/ISBN: 0001-6764 PMID: 214996 Document Number: 113705
A convenient method for purifying RNA tumor viruses is described. Viruses banded in isopycnic gradients are contaminated with membranes that can be removed by velocity sedimentation in glycerol gradients. On sodium dodecyl sulfate (SDS) gel electrophoresis, the particles purified by these procedures from mouse mammary tumor tissue show protein profiles typical of those observed with virus purified from milk.