Trial of purifying tumor degenerating factor of human fibroblasts
Imanishi, J.; Uemura, H.; Tanaka, A.; Matsuoka, H.; Kakui, Y.; Imanishi, T.; Nishino, H.; Kishida, T.
Comptes Rendus des Seances de la Societe de Biologie et de ses Filiales 178(3): 313-316
1984
ISSN/ISBN: 0037-9026 PMID: 6238658 Document Number: 221380
Tumor-degenerating factor (TDF) with the specific activity of 2.9 units/mg of protein was produced and purified by several chromatographies. The specific activity was increased to 302 units/mg of protein by DEAE-Sephadex A-50 chromatography, repeated twice. Then, this preparation was purified to the specific activity of 2,040 units/mg of protein with recovery rate of 16.6% by Con A-Sepharose and CM-Sephadex C-50 chromatographies. Finally, the specific activity was increased to 9,010 units/mg of protein with the final recovery rate of 14.6% by Blue Sepharose CL-6B chromatography.