Histone modification by 1,3-diphosphoglycerate: site of acylation and quantitative estimation
Manao, G.; Camici, G.; Cappugi, G.; Ramponi, G.
Physiological Chemistry and Physics 9(2): 117-122
1977
ISSN/ISBN: 0031-9325 PMID: 601105 Document Number: 113481
A quantitative method for determining 3-phosphoglyceric acid bound to f1-histone during 1,3-diphosphoglycerate-histone interaction is described. The method is based on gas chromatographic analysis of the glyceric acid split from histone by acid hydrolysis. Evidence that .epsilon.-NH2 groups can be acylated by 1,3-diphosphoglycerate is presented.