Comparative evaluation of acute and subacute toxic action of aniline and O-isopropoxyaniline
Czajkowska, T.; Krysiak, B.; Stetkiewicz, J.
Medycyna Pracy 28(3): 157-174
1977
ISSN/ISBN: 0465-5893 PMID: 895504 Document Number: 113385
The comparison of acute and subacute toxic action of aniline and o-isopropoxyaniline (o-IPA) has been carried out. In the course of acute experiment, the following results were obtained: DL50 values for aniline and o-IPA established for rats after administration by stomach-tube were--0,45 g/kg and 0,84 g/kg, after intraperitoneal administration--0,34 g/kg and 0,23 g/kg, after superficial coating of the skin surface--0,67 g/kg and 2,20 g/kg body weight, respectively. O-IPA, similarly as aniline, exerts a methemglobinopoietic effect. The both substances have local irritant effect on the skin and the eye of rabbit, causing reactions of middle degree of intensity. The examinations of aniline and o-IPA, performed after Lima's method, have revealed no comulataive action. During the 6 months exposure consisting in administration to animals of doses amounting to 1/10 and 1/2 of DL50 value by stomach tube, changes in the picture of peripheral blood were found as well as statistically significant increase of phenol red urine excretion, the inhibition of body weight increase and statistically significant increase of relative and absolute weight of liver and spleen. The both compounds damage the liver and kidneys parenchyma. The degree of liver injury is greater due to aniline action, o-IPA, however, has a stronger action on the excretion of renal cortex epithelium. In the spleen, a similar type of changes was observed, but the number of erthroblasts and reticuloendothelial system cells as well as the degree of hemosyderine deposition in this organ was greater after aniline administration. These results demonstrate that the toxic character of o-IPA and of aniline is similar, but the changes provoked by o-IPA are less intensive.