A discrete multichanneal procedure for the chemical determination of urinary estrogens during pregnancy
Penttilä, I.M.; Jokela, H.; Puhakainen, E.; Nummi, S.; Rantanen, T.; Viitala, A.J.
Journal of Clinical Chemistry and Clinical Biochemistry 15(5): 271-274
1977
ISSN/ISBN: 0340-076X PMID: 894195 Document Number: 112514
A colorimetric method, based on the Kober reaction, was adapted for the determination of urinary estrogens during pregnancy. The method yields final volumes for direct measurements by a multichannel computer-controlled photometer. The speed of the analysis was increased, so that 100 samples can easily be completed by 1 laboratory technician as triplicate analyses during 1 working day. A satisfactory accurracy and precision of the method were also achieved. The values were determined using urine samples from 1097 apparently healthy pregnant women.