Semiautomated method for the fluorometric determination of total vitamin C in food products
Egberg, D.C.; Potter, R.H.; Heroff, J.C.
Journal - Association of Official Analytical Chemists 60(1): 126-131
1977
ISSN/ISBN: 0004-5756 PMID: 833086 Document Number: 111587
A simple method employing simultaneous extraction and oxidation has been developed for the semiautomated determination of ascorbic and dehydroascorbic acids in food products. Recovery studies were conducted on ready-to-eat breakfast cereals and both fresh and canned fruits and vegetables, with average recoveries of 101, 100, and 102%, respectively. Reproducibility data were generated showing a relative standard deviation of 3.5%. The automated method was compared with the manual AOAC fluorometric method and with indophenol titration ; correlation coefficients were 0.9960 and 0.9926, respectively. The hydrolysis product of dehydroascorbic acid, 2,3-diketoguIonic acid, a reported interference in this method, was prepared and shown not to form an interfering fluorescent derivative.