Simple and fast fluorometric method for the determination of glycine (or any primary amine) in dietetic beverages
Coppola, E.D.; Hanna, J.G.
Journal - Association of Official Analytical Chemists 57(6): 1265-1268
1974
ISSN/ISBN: 0004-5756 PMID: 4430643 Document Number: 79756
The reagent fluorescamine (4-phenylspiro- (furan-2 (3H),l´-phthalan)-3,3´-dione), which reacts selectively with primary amines in alkaline solution, has been used to determine glycine in dietetic beverages in a simple and fast fluorometric procedure. As little as 0.02% glycine can be determined. An average recovery of 100.3±3.76% was obtained for added glycine in a series of 9 determinations. The method can be used as a screening procedure to confirm or exclude the presence of glycine in dietetic beverages.