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A rapid sensitive radioassay for serum native and denatured DNA

Cox, R.A.; Gokcen, M.

Research Communications in Chemical Pathology and Pharmacology 17(2): 309-318

1977

ISSN/ISBN: 0034-5164
PMID: 877415
Document Number: 110887
A radioassay for human serum DNA using 3H-actinomycin D was developed and evaluated. The assay was found to be technically simple, precise, sensitive and specific for DNA. Both native and denatured DNA levels could be measured using a nuclease specific for sDNA, since denatured DNA reproducibly bound 3H-actinomycin D under the assay conditions. Analysis of human sera yielded results in accord with those previously reported for man.

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