A rapid and sensitive method for detecting human cells in xenogeneic hosts
Zompa, E.A.; Moyer, M.P.; Cain, L.D.; Bottenstein, J.E.; Hulsebosch, C.E.
Transplantation 66(7): 928-931
1998
ISSN/ISBN: 0041-1337 PMID: 9798706 Document Number: 495190
Xenografts, specifically transplantation of human cells into other species, are a valuable tool in preclinical transplantation experiments. A central issue is accurate identification of the grafted cells, particularly in cases in which cellular migration has occurred. We report that detection of grafted human cells can be achieved by in situ hybridization techniques using human centromeric probes which result in unambiguous nuclear labeling. The resulting reaction can be combined with immunocytochemical or histochemical techniques for cell-type characterization.