Immunologic study of the cellular components of pseudomonas pyocyaneus. II. Extraction of purified lipopolysaccharide, its characteristics and elaboration of an erythrocyte O-diagnosticum
Kolker, I.I.; Stanislavskiĭ, E.S.; Zarubina, E.K.; Elagina, E.K.; Kaliaev, A.V.
Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 12: 113
1976
ISSN/ISBN: 0372-9311 PMID: 828824 Document Number: 108906
The authors elaborated a method of obtaining a highly active lipopolysaccharide preparation from the cell membranes of the Pseudomonas aeruginosa whose sensitizing activity exceeded that of the polysaccharide isolated from the intact microbial cells almost 20 times. The optimal sensitizing dose of the preparation constituted 15 micron/ml. An erythrocytic diagnostic agent permitting to determine the antibodies in the sera of patients with the purulent-in flammatory diseases caused by Ps. aeruginosa was prepared on the basis of the purified lipo polysaccharide. A definite dependence of the hemagglutinin titres on the severity of the affection and the degree of the purulent-inflammatory process was revealed.