Dimethyl sulfoxide-induced enhancement of 7,12-dimethylbenz (a) anthracene metabolism and DNA binding in differentiating mouse epidermal cell cultures
Yuspa, S.H.; Hennings, H.; Dermer, P.; Michael, D.
Cancer Research 36(3): 947-951
1976
ISSN/ISBN: 0008-5472 PMID: 814999 Document Number: 108783
Mouse epidermal cells in primary culture differentiate rapidly over a 2-week period leading to keratinization and sloughing of most of the plated cells. Cell replication was partially synchronized in these cultures with peaks of DNA synthesis at the 2nd and 8th day. The ability of epidermal cells to metabolize 7,12-dimethylbenzanthracene binding to epidermal DNA was two- to fourfold lower in 10-day than 3-day cultures. This decrease in metabolism and binding between 3-day and 10-day cultures could be eliminated by the inclusion of 1.25% dimethyl sulfoxide in the culture medium during the entire culture period.