In vitro correlates of transformation in C3H/10T1/2 clone 8 mouse cells
Jones, P.A.; Laug, W.E.; Gardner, A.; Nye, C.A.; Fink, L.M.; Benedict, W.F.
Cancer Research 36(8): 2863-2867
1976
ISSN/ISBN: 0008-5472 PMID: 1277196 Document Number: 108743
Various potential in vitro correlates of malignancy were studied in four chemically transformed C3H/10T1/2 Clone 8 mouse cell lines and were compared with controls cells. The degree of tumorigenicity was best predicted by the relative plating efficiencies of the morphologically transformed cells in soft agar. All transformed cells also showed an increase in extracellular fibrinolytic activity which may be an additional marker for transformation. Intracellular fibrinolytic activity and loss of 125I-labeled cell surface protein (M.W. 250,000) were not correlated with morphological transformation or tumorigenicity in these cells.