Study of collagen sponge extracts on mouse splenic lymphocyte transformation in vitro
Wu, S.; Liu, C.; Hou, L.; Sun, X.; Gai, X.; Shi, Y.
Zhongguo Yi Liao Qi Xie Za Zhi 38(4): 308-311
2014
ISSN/ISBN: 1671-7104 PMID: 25330619 Document Number: 678695
Immunogenicity for medical devices of animal origin is the key and difficult point during immune safety evaluation for these devices. This paper firstly investigated the effect of collagen sponge of animal origin on mouse splenic lymphocyte transformation and proliferation, and then analyzed the influence factors on the MTT method and CFSE method. The results showed that collagen sponge extract cannot significantly induce transformation and proliferation of mouse splenic lymphocyte in vitro.