The effect of anions on Mg-ATPase in red blood cells
Mircevová, L.; Simonová, A.
Acta Physiologica Academiae Scientiarum Hungaricae 48(1): 51-57
1976
ISSN/ISBN: 0001-6756 PMID: 146397 Document Number: 108641
Anions exert an influence on the passive permeability of Na+ and K+ in erythrocytes. THE EFFECT ON Mg-ATPase activity has been studied in human erythrocytes. 40 mM bicarbonate increased the activity as compared to the effect of 40 mM chloride; 20 mM sulphate inhibited it. Salicylate acted first as an activator then as an inhibitor of Mg-ATPase; maximum activity was reached at 60 mM CONCENTRATION. Thiocyanate inhibited saponin-stimulated Mg-ATPase, Ki = 1.85 X 10(-2)M. The probable mechanisms of action of the above anions on Mg-ATPase and possible relation to passive permeability of Na+ and K+ ions are discussed.