Effect of 2,4-diamino-5-chlorphenyl-6-ethylpyrimidine on the oncorporation of P32-phosphate into adenylic nucleotides of rat embryo and placenta
Kotin, A.M.; Chebotar', N.A.
Biokhimiia 41(5): 801-806
1976
ISSN/ISBN: 0320-9725 PMID: 1030636 Document Number: 108411
Teratogenic doses of antimalaria preparation, 2,4-diamino-5-p-chlorophenyl-6-ethylpyrimidine (chloridine), known in biochemistry as a specific inhibitor of dihydrofolate reductase, result in the decrease of 32P-phosphate incorporation into ATP and ADP, coupled with the inhibitions of DNA synthesis, in rat embryo and placenta on 13th day of development, while the incorporation of 14C-formiate into free nucleotides of acid soluble embryo and placenta fractions (at the same intervals after teratogen injections), is the same as in the control. These data show that the primary blocking of folate cycle and DNA biosynthesis in 13 days old embryos with induced anomalies of development is some way coupled with disturbances of ATP metabolism.