Determination of thiazinamium sulphoxide in urine by means of amberlite XAD-2 column chromatography and thin-layer densitometry
Jonkman, J.H.; Wijsbeek, J.; Greving, J.E.; Van Gorp, R.E.; De Zeeuw, R.A.
Journal of Chromatography 128(1): 208
1976
ISSN/ISBN: 0021-9673 PMID: 993299 Document Number: 104751
The application of Amberlite XAD-2 resin for the isolation from human urine of the quaternary metabolite thiazinamium sulfoxide, the main metabolite of the anticholinergic drug thiazinamium methylsulfate (Multergan) is reported. After the isolation 2-dimensional TLC was used for separation of the parent drug and the metabolite. The spots are visualized by immersion in an oxidative mixture and quantitation is done by measuring transmission, using an in situ method. The detection limit for the procedure is 40 ng/ml in urine. Accurate quantitation can be achieved at concentrations of 250 ng/ml and above. The recovery at a therapeutic concentration of 10 .mu.g/ml in urine was 90.6 .+-. 6.5% (n = 7).