A rapid and sensitive method for the determination of the lithogenic index of bile by thin-layer chromatography and densitometry using a dual-wavelength chromatoscanner
Kibe, A.; Kuramoto, T.; Hoshita, T.
Analytical Biochemistry 100(1): 146-151
1979
ISSN/ISBN: 0003-2697 PMID: 543533 Document Number: 148848
A rapid and sensitive method for quantitative determination of 3 bile lipid components, cholesterol, bile acids, and lecithin, is described. These components were separated by TLC on a silica gel plate, spots were visualized with a phosphomolybdate reagent and their color intensities were estimated by direct densitometry using a dual-wavelength chromatoscanner. The lithogenic index was estimated by the molar ratio of the 3 lipids. This method can be applied to the routine analysis of bile in patients with gallstones. It was evaluated by comparison with the method using standard GLC and spectrophotometry.