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Transcription of repeated and unique DNA nucleotide sequences in rat thymus cells stimulated with phytohemagglutinin

Khanson, K.P.; Zhivotovskiĭ, B.D.

Biokhimiia 41(11): 2031-2036

1976

ISSN/ISBN: 0320-9725
PMID: 1022272
Document Number: 103558
Transcription of unique and repeated DNA sequences in normal and phytohemagglutinin (PHA)-stimulated rat thymus cells is studied. It is found that heterogenous nuclear RNA (hnRNA) contains the transcript both from unique and repeat-d DNA sequences. PHA does not induce re-distribution of hnRNA fractions transcripted from different for their recurrency genome regions. Moreover, hnRNA from normal thymus cells competes with RNA from stimulated cells within the range of COt values (10(-3)-10(-4)) in which the hybridization has been carried out. It is concluded that no activation of new genome regions takes place in thymocytes.

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