Physiological dependency between the hypothalamus and the thymus in Wistar rats. III. Hypothalamic cells cultured in vitro in the presence of Thymus Factor X (TFX) and fresh thymus filtered homogenate
Ficek, W.
Gegenbaurs Morphologisches Jahrbuch 129(1): 112-119
1983
ISSN/ISBN: 0016-5840 PMID: 6682814 Document Number: 216162
The studies were carried out on the hypothalamic cells cultured in vitro in the presence of low-molecular proteins (constituting the control): the thymic hormone, known as Thymus Factor X [TFX]; or fresh thymus filtered homogenate prepared from the glands collected from 1 h or 7 d old rats. All the cultures were supplemented with 3H-thymidine. The experiments demonstrated that TFX in when added to the culture caused a decrease 3H-thymidine synthesis in comparison to the control. In other cultures, which were enriched with the fresh thymus filtrated homogenate, especially from 7 d old rats, a high level of thymidine synthesis in the cellular nuclei was observed.