Immunochemical studies of the plasma and cultured fibroblast media fractions containing the cystic fibrosis ciliary inhibitor
Harper, B.L.; Barnett, D.R.; Bissett, J.D.
Texas Reports on Biology and Medicine 34(1): 73-82
1976
ISSN/ISBN: 0040-4675 PMID: 186909 Document Number: 102406
The cystic fibrosis ciliary inhibitor (CFCI) was fractionated from plasma of (but not C3a), C4, C5 .alpha.1-lipoprotein, .beta.-lipoprotein, .beta.2-microglobulin and immunoglobulin L chains were detected by hemagglutination in fractions of CF plasma that inhibited ciliary activity and in analogous fractions from normal sera. None of the proteins were detected in media of cultured fibroblasts from either genotype. Since the same proteins and protein fragments were identified in CF and normal plasma fractions, and were not detected in CF fibroblast media, it appears that none of these proteins can be identified as the CFCI. Identification of these proteins will permit further purification of the CFCI by immunochemical methods.