Antibody response to Chandipura virus in experimental animals
Kelkar, S.D.
Indian Journal of Medical Research 64(6): 814-823
1976
ISSN/ISBN: 0019-5340 PMID: 824199 Document Number: 102020
The antibody response to Chandipura (CHP) virus was studied in different laboratory animals, (mice, guinea-pigs, rabbits, monkeys and chickens) by complement fixation (CF), neutralization (N) and agar gel diffusion (AGD) tests. The CF antibody response was good in guinea-pigs and monkeys, poor in rabbits and negligible in chickens. The neutralizing (N) and agarose gel precipitating (AGP) antibodies appeared in all the animals. The N antibodies appeared 11 days earlier than the CF antibodies in mice and 3 days earlier in guinea-pigs and monkeys. The time of appearance of AGP antibodies coincided with that of the CF antibodies in most cases. The presence of short-lived ethanethiol sensitive CF antibodies in the case of guinea-pigs and 1 rabbit and 2-mercaptoethanol (2-ME) sensitive N antibodies in the case of guinea-pig and monkey sera was demonstrated. The guinea-pigs and monkeys appeared to be the animals of choice to prepare high titers immune sera by single inoculation of CHP virus.