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Comparison of batch and chromatographic assays of cyclic nucleiotide phosphodiesterases

Gulyassy, P.F.; Farrand, J.R.

Journal of Chromatography 129: 107-113

1976

ISSN/ISBN: 0021-9673
PMID: 187607
Document Number: 101899
An improved method for the assay of cyclic nucleotide phosphodiesterases (CNPases) is described in which residual 3H-cAMP or 3H-cGMP is separated from products by chromatography on thin layers of PEI-cellulose. Comparison of CNPase activity in crude or purified rat kidney enzyme assayed by the PEI-cellulose method with a batch anion-exchange resin method showed serious underestimation of activity by the latter method. The batch method underestimated CNPase activity by 34-82%. The error was due to substantial binding of nucleosides (adenosine and guanosine) to the resin, which is assumed not to occur by those using this method.

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Robberecht, P.; Deschodt-Lanckman, M.; De Neef, P.; Christophe, J. 1973: Cyclic nucleotide phosphodiesterases in rat pancreas Archives Internationales de Physiologie et de Biochimie 81(3): 593
Haslam, R.J.; Dickinson, N.T.; Jang, E.K. 1999: Cyclic nucleotides and phosphodiesterases in platelets Thrombosis and Haemostasis 82(2): 412-423
Stefanovich, V.; Ambrus, J.L.; Ambrus, C.M.; Karakousis, C.; Takita, H. 1981: Cyclic nucleotide phosphodiesterases in normal and malignant human tissues Journal of Medicine 12(4): 243-250
Sharagina, L.M.; Savvateeva, E.V.; Atamanenko, A.A. 1997: Cyclic nucleotide phosphodiesterases in mutant lines of Drosophila melanogaster Genetika 33(6): 784-787
Stefanovich, V. 1975: Inhibition of platelet cyclic 3',5'-adenosine-monophosphate phosphodiesterases by pentifylline Arzneimittel-Forschung 25(5): 740-741
Pickering, L.K.; Hoecker, J.L.; Kramer, W.G.; Liehr, J.G.; Caprioli, R.M. 1979: Assays for chloramphenicol compared: radioenzymatic, gas chromatographic with electron capture, and gas chromatographic-mass spectrometric Clinical Chemistry 25(2): 300-305
Medvedeva, M.V. 1995: Modern representations of multiple forms of cyclic nucleotide phosphodiesterases in mammalian tissues Biokhimiia 60(3): 364-386
Lugnier, C.; Doré, J.C.; Stoclet, J.C.; Viel, C. 1992: Utilization of a multiparametric technique for analysis of specificity of different inhibitors of various forms of cyclic nucleotide phosphodiesterases Die Pharmazie 47(1): 46-49
Wells, J.N.; Wu, Y.J.; Baird, C.E.; Hardman, J.G. 1975: Phosphodiesterases from porcine coronary arteries: inhibition of separated forms by xanthines, papaverine, and cyclic nucleotides Molecular Pharmacology 11(6): 775-783
Ng, W.J.; Ong, S.L.; Tan, K.Y.; Hu, J.Y.; Lee, L.Y.; Song, L.F. 2002: Toxicity assays to determine the start-up strategy for an anaerobic sequencing batch reactor (anSBR) Water Science and Technology: a Journal of the International Association on Water Pollution Research 46(11-12): 343-348
Onali, P.; Strada, S.J.; Chang, L.; Epstein, P.M.; Hersh, E.M.; Thompson, W.J. 1985: Purification and characterization of high-affinity cyclic adenosine 5'-monophosphate phosphodiesterases from human acute myelogenous leukemic cells Cancer Research 45(3): 1384-1391
Schwabe, U.; Miyake, M.; Ohga, Y.; Daly, J.W. 1976: 4-(3-Cyclopentyloxy-4-methoxyphenyl)-2-pyrrolidone (ZK 62711): a potent inhibitor of adenosine cyclic 3',5'-monophosphate phosphodiesterases in homogenates and tissue slices from rat brain Molecular Pharmacology 12(6): 900-910
McDougal, J.S.; Hubbard, M.; Strobel, P.L.; McDuffie, F.C. 1982: Comparison of five assays for immune complexes in the rheumatic diseases: performance characteristics of the assays Journal of Laboratory and Clinical Medicine 100(5): 705-719
Wakabayashi, H.; Cook, D.J.; Posner, M.; King, A.; Mendez-Picon, G.; Brown, K.; Lee, H.M. 1991: Batch-to-batch variations in rabbit antithymocyte globulin preparations Transplantation Proceedings 23(1 Pt 2): 1125-1127
Chiou, W.L.; Peng, G.W.; Nation, R.L. 1977: Ethanol as a mobile-phase solvent in high-performance liquid-chromatographic assays Clinical Chemistry 23(12): 2355
Van der Wal, S.; Snyder, L.R. 1981: Precision of "high-performance" liquid-chromatographic assays with sample pretreatment. Error analysis for the Technicon "FAST-LC" system Clinical Chemistry 27(7): 1233-1240
Masucci, J.J.A.; Caldwell, G.G.W.; Jones, W.W.J.; Juzwin, S.S.J.; Sasso, P.P.J.; Evangelisto, M. 2001: The use of on-line and off-line chromatographic extraction techniques coupled with mass spectrometry for support of in vivo and in vitro assays in drug discovery Current Topics in Medicinal Chemistry 1(5): 463-471
Rosenberg, A.M.; Prokopchuk, P.A. 1988: Comparison of ANA testing in JRA using immunofluorescent and immunoenzyme assays on HEp-2 cells Journal of Rheumatology 15(1): 148-149
Mora-Brugués, J.; Gascón-Roche, N.; Rodríguez-Espinosa, J.; González-Sastre, F. 1994: Comparison of two serum alpha-fetoprotein assays: Amerlite AFP and AFP-2T Annales de Biologie Clinique 52(6): 467-468
Ben Moussa, M.; Barguellil, F.; Bouziani, A.; Amor, A. 2003: Comparison of two hepatitis C virus typing assays in a Tunisian population Annales de Biologie Clinique 61(2): 234-238