Effect of diet high in beef fat on the composition of fecal bile acids during intestinal carcinogenesis in the rat
Nigro, N.D.; Campbell, R.L.; Singh, D.V.; Lin, Y.N.
Journal of the National Cancer Institute 57(4): 883-888
1976
ISSN/ISBN: 0027-8874 PMID: 1003534 Document Number: 100835
Two groups of 20 male Sprague-Dawley rats were given as carcinogen azoxymethane 8 mg/kg bodyweight subcutaneously and fed on a normal diet or one with 35% beef fat. Control groups were not given azoxymethane. Fat-control rats did not excrete more total bile acids than the normal-control group but excreted more deoxycholic acid as the result of increased cholic acid degradation. Azoxymethane itself increased faecal bile acid concentration but tended to reduce cholic acid degradation. Fatty acid content in the faeces increased in rats on the fat diet but was not affected by azoxymethane. An increase dependent on fat diet was apparent in total faecal neutral steroids and a carcinogen-dependent increase in cholesterol degradation. Dietary fat and bile steroids altered by gut microflora were interrelated in the intestinal carcinogenic process.