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A fluorescent method of determining aldosterone in urine

Botvin'ev, O.K.; Atamanova, T.M.

Problemy Endokrinologii 22(3): 44-47

1976

ISSN/ISBN: 0375-9660
PMID: 935087
Document Number: 99910
The authors suggest a method of aldosterone determination in the urine. Aldosterone is extracted from the sample and purified with the aid of columnar and thin-layer chromatography on silicagel. Aldosterone is distinctly identified in ultraviolet light on the chromatogram by its green fluorescence developing after sprinkling the plate with phosphoric acid and its subsequent heating. Quantitative determination is carried out by the measurement of the intensity of aldosterone fluorescence in sulfuric acid.

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