Characteristics and use of the "ROTOP-insulin-test'' for radioimmunologic determination of insulin
Ziegler, M.; Shultz, B.; Gottschling, D.; Keilacker, N.; Michaelis, D.
Problemy Endokrinologii 22(3): 51-54
1976
ISSN/ISBN: 0375-9660 PMID: 935088 Document Number: 97753
The authors describe characteristics and clinical application of the "ROTOP-insulin-test'' set. Radioimmunological insulin determination is carried out by the principle of reverse titration: 50 ml of plasma is incubated for 18 hours with antiserum. The remaining free valencies of insulin antibodies are bound with the excess of 125I-insulin. Bound 125I-insulin is precipitated after the addition of alcohol. The variation coefficient is 7.4% in one batch and 9.1% in analysis in 48 hours (insulin concentration = 2.08 ng/ml = 49 mcU/ml). The precision index of the standard curve was 0.022. Insulin secretion was analyzed in 107 healthy persons with a normal body weight under conditions of prolonged glucose infusion. Two phases of insulin secretion were shown.