Lysosome-like alpha-granules of thrombocytes: detection by acridine orange treatment, properties and transformations during the course of blood coagulation reactions
Kozlov, V.K.
Tsitologiia 17(7): 762-767
1975
ISSN/ISBN: 0041-3771 PMID: 51527 Document Number: 95153
Using the ability of lysosome-like alpha-granules of thrombocytes to accumulate flurochrome acridine orange, their transformation kinetics was studied in the course of cell viscous metamorphosis utilizing the fluorometric technique. It turned out that during the thrombin induced cellular viscous metamorphosis lysosomes released their contents: the process is stimulated by the presence of extracellular Ca2+. Competitive relations were revealed between the accumulation within cytoplasmic granules of thrombocytes of acridine orange and serotonin, and that of histamine and novocaine. The process of fluorochrome accumulation was found to be temperature dependent and to be inhibited by monoiodacetate.