Change in the fluorescent characteristics of rat lymphocytes stained with acridine orange after acute gamma-irradiation
Karnaukhova, N.A.; Sergievich, L.A.; Aksenova, G.E.
Biofizika 39(1): 123-128
1994
ISSN/ISBN: 0006-3029 PMID: 8161582 Document Number: 431023
Changes of parameter alpha of rat blood lymphocytes after acute gamma-irradiation have been investigated by luminescence microspectral analysis. Parameter alpha reflects the cell synthetic activity and represents the ratio of intensities of the red (640 nm) and green (530 nm) components the luminescence spectrum of acridine orange fluorochromed cells. It has been demonstrated that changes of parameter alpha occur in three stages: a) sharp decrease at the 1st or 2nd day after irradiation: b) increase with the maximum at the 4th-15th day; c) fluctuations on the level higher then the control values. At each stage there exist typical specific shapes of parameter alpha distribution histograms. These results indicate that this method may be used for control of the immune system state during gamma-radiation sickness or in the process of human cancer radiation therapy.