Methods of obtaining antisera against antigens of the mammary cancer virus
Kriukova, I.N.; Miller, G.G.; Frolova, T.I.
Biulleten' Eksperimental'noi Biologii i Meditsiny 80(9): 122-125
1975
ISSN/ISBN: 0365-9615 PMID: 177127 Document Number: 94816
The authors used various methods of producing virus-containing preparations from mouse mammary carcinoma to obtain rabbit antisera against the mammary carcinoma antigens. The best results were achieved by means of the virus precipitation from the cultural fluids of carcinomas growing in vitro after preliminary hormonal stimulation of the virus yield followed by its purification in the sucrose density gradient.
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