Necrotaxis: a scanning and transmission electron microscopy study
Vethamany, V.G.; Vethamany, S.K.; Bessis, M.
Nouvelle Revue Francaise d'Hematologie 15(1): 83-98
1975
ISSN/ISBN: 0029-4810 PMID: 126426 Document Number: 94596
This paper describes an easy method to determine a "necrotactic rosette forming index (NRI) of leucocytes" after destruction of a red cell by a laser beam. Among the drugs tested, only cytochalasin B (0.5 mug/ml) is found to significantly lower NRI. The lectins PHA and CON-A did not have any effect on the NRI. Patients with infections are found to have increased NRI. Transmission and scanning electron microscopy have permitted the study of fine structurale details of the entire phenomenon.